Suhayli A. Muminjonov, Shuhrat F. Odinaev, Bakhtovar J. Karim-zade, Islomuddin A. Yunusov
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Cellular Therapy and Transplantation (CTT)
Volume 6, Number 3
The study aimed to evaluate safety and effectiveness of autologous hemopoetic bone marrow stem cell transplantation in post-infarction cardiosclerosis, using intracoronary injection of CD133+ cells.
Patients and methods
The study included 30 patients at mean age of 52 ± 8 years. All the patients were treated for their heart disorder using a conventional protocol, being divided into two groups, i.e., control and stem cell-transplanted group. 15 patients in stem cell transplanted group were treated by the CD133+ stem cells and supplementary therapy with estradiol (0.75 mg/day for 2 months). Bone marrow samples were taken according from the sternum by a standard puncture. CD133+ stem cells were isolated by density gradient centrifugation in Ficoll followed by immuno-magnetic separation. Autologous isolated cells of patients (5 ml of suspension containing 0.8 to 1.5x106 cells) were used for each patient with coronary artery disease; the cells were injected into coronaries during angiography. Clinical investigations such as LVEF (left ventricle ejection fraction of the), ESV (end-systolic volume) and EDV (end-diastolic volume) were performed. Scintigraphy was carried out in 11 of 15 treated patients with stem cell transplantation before the treatment, and 1, 3 and 9 months after the cell therapy, in order to evaluate the dynamics of myocardial perfusion in these patients.
After the stem cell therapy, EFLV vakues were increased from 50 ± 9% to 58 ± 9% at 6 months after treatment. Similar indicators of EFLV were observed in control group. No significant difference in hemodynamic parameters was found between the two groups. Positive results of heart regeneration in stem cell transplanted group were observed by myocardial scintigraphy using Tc99m with the methoxyisobutylisonitrile (MIBI) marker ligand.
The procedure for implantation of autologous stem cells CD133 isolated from the bone marrow of the patients with post-infarction cardiosclerosis is safe, has a beneficial effect upon the process of rehabilitation treatment of cardiovascular diseases.
Stem cells, CD133+, post infarction cardiosclerosis, bone marrow stem cells, MIBI marker.