OID-04. Immunogenetic profile of the Russian and Belarusian hematopoietic stem cells donors registries
Ludmila N. Bubnova1, Elena V. Kuzmich1, Alena V. Hlaz2, Irina E. Pavlova1, Maria A. Terentyeva1, Anatoly L. Uss2, Ihar A. Iskrou2, Siarhei P. Liashchuk2
1 Russian Research Institute of Hematology and Transfusiology, St. Petersburg, Russia
2 State Institution “Minsk Scientific and Practical Center for Surgery, Transplantology and Hematology”, Minsk, Republic of Belarus
Contact: Dr. Elena V. Kuzmich, phone: +7 (921) 912-52-07, e-mail: yelenakuzmich@gmail.com
Summary
Collaboration between bone marrow donor registries of different countries increases the chances of HLA-compatible unrelated donor selection for patients in need of allogeneic hematopoietic stem cell transplantation without available donors in registries of the country of residence. The donor search success and duration depends on patients’ HLA-haplotypes frequency, as well as patient’s ethnic group representatives in the registry. We performed a comparative analysis of the profile of HLA-A*-B*-C*-DRB1*-DQB1*-haplotypes of potential hematopoietic stem cells (HSC) donors of the FSBI RosNIIHT FMBA of Russia and the Republic of Belarus registries.
Materials and methods
The study included 2853 potential HSC donors of FSBI RosNIIHT FMBA of Russia registry. Primary immunogenetic examination was performed using PCR-SSP (“Protrans”, Germany) and PCR-SSOP (“BAG HEALTH CARE”, Germany). The surveyed group of potential HSC donors of the registry of the Republic of Belarus included 2420 people. PCR-SSOP (“Immucor”, USA) and PCR-SSP (“One Lambda”, “Olerup”, USA) were used for primary HLA-typing of donors. The HLA haplotypes frequencies were determined by maximum likelihood method using the EM algorithm provided by Arlequin 3.5 software.
Results
A total of 1702 HLA-haplotypes were identified in the FSBI RosNIIHT FMBA of Russia cohort examined. The most common HLA-haplotypes were: A*01-B*08-C*07-DRB1*03-DQB1*02 (0,0366), A*03-B*07-C*07-DRB1*15-DQB1*06 (0,0269), A*03-B*35-C*04-DRB1*01-DQB1*05 (0,0238), A*02-B*13-C*06-DRB1*07-DQB1*02 (0,0204), A*02-B*07-C*07-DRB1*15-DQB1*06 (0,0184), A*25-B*18-C*12-DRB1*15-DQB1*06 (0,0127), A*02-B*18-C*07-DRB1*11-DQB1*03 (0,0126), A*02-B*15-C*03-DRB1*04-DQB1*03 (0,0123), A*02-B*41-C*17-DRB1*13-DQB1*03 (0,0109), A*30-B*13-C*06-DRB1*07-DQB1*02 (0,0099). Also, 1365 HLA-haplotypes were determined in the Republic of Belarus potential HSC donors registry. The most common HLA-haplotypes were: A*01-B*08-C*07-DRB1*03-DQB1*02 (0,0445), A*03-B*07-C*07-DRB1*15-DQB1*06 (0,0330), A*02-B*13-C*06-DRB1*07-DQB1*02 (0,0294), A*02-B*07-C*07-DRB1*15-DQB1*06 (0,0194), A*03-B*35-C*04-DRB1*01-DQB1*05 (0,0179), A*02-B*18-C*07-DRB1*11-DQB1*03 (0,0159), A*25-B*18-C*12-DRB1*15-DQB1*06 (0,0141), A*02-B*57-C*06-DRB1*07-DQB1*03 (0,0118), A*11-B*35-C*04-DRB1*01-DQB1*05 (0,0112), A*23-B*44-C*04-DRB1*07-DQB1*02 (0,0109), A*02-B*27-C*02-DRB1*16-DQB1*05 (0,0105). The comparative analysis revealed similarities as well as differences in HLA-haplotypes distribution among donors of Belarusian and Russian registries. In particular, the frequencies of A*02-B*13-C*06-DRB1*07-DQB1*02, A*02-B*57-C*06-DRB1*07-DQB1*03, A*02-B*27-C*02-DRB1*16-DQB1*05 haplotypes are significantly higher in donors of Belarus Republic registry (p=0.04; 0.01 and 0.01 respectively). The frequency of A*02-B*41-C*17-DRB1*13-DQB1*03 haplotype is considerably higher in donors of FSBI RosNIIHT FMBA of Russia registry (p=0.03).
Conclusions
The HLA-A*-B*-C*-DRB1*-DQB1*-haplotypes profiles of potential Belarusian and Russian registries HSC donors have characteristic features. Using united donor resources for donor search will increase patient’s chances for optimal unrelated donors selection in term of their immunogenetic characteristics.
Keywords
Donor registry, hematopoietic stem cells, HLA-haplotypes.
