ISSN 1866-8836
Клеточная терапия и трансплантация

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Experimental studies

Therapeutic potential of mouse bone marrow mesenchymal stromal cells transplantation in ameliorating of CCL4-induced liver damage (in vivo study)

Dima Joujeh1, Abduljalil Ghrewaty1, Chadi Soukkarieh2, Adnan Almarrawi1, Jamal Abdul Naser Darwicha3

Experimental studies

Therapeutic potential of mouse bone marrow mesenchymal stromal cells transplantation in ameliorating of CCL4-induced liver damage (in vivo study)

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Dima Joujeh1, Abduljalil Ghrewaty1, Chadi Soukkarieh2, Adnan Almarrawi1, Jamal Abdul Naser Darwicha3

1 Department of Biotechnology Engineering, Faculty of Technical Engineering, University of Aleppo, Syria
2 Department of Animal Biology, Faculty of Sciences, Damascus University, Damascus, Syria
3 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Arab International University, Syria


Correspondence:
Dr. Dima Joujeh, Department of Biotechnology Engineering, Faculty of Technical Engineering, University of Aleppo, Syria
Phone: (+963) 994-046-745
E-mail: dimajoujeh@gmail.com


Citation: Joujeh D, Ghrewaty A, Soukkarieh C et al. Therapeutic potential of mouse bone marrow mesenchymal stromal cells transplantation in ameliorating of CCL4-induced liver damage (in vivo study). Cell Ther Transplant 2022; 11(1): 58-68.

Liver diseases pose a major global health problem. In this study, the effect of bone marrow derived mesenchymal stromal cells MSC(M) in liver damage induced by CCl4 treatment was investigated.

Materials and methods

Balb/c mice were injected by (1 ml/kg body weight) of CCl4 twice a week for 7 weeks. The mice were then intravenously injected with MSC(M) (at the passage 0), or physiological saline (PBS, control group). Four weeks after transplantation, serum levels of ALT, AST, ALP and albumin were measured, liver histopathology was performed, and ELISA technique was carried out to detect the serum levels of IL-4 and IFN-γ.

Results

As compared with PBS-treated animals, MSC(M)- treated group presented with lower levels of ALT and ALP, being also associated with less pronounced changes in serum albumin. Histological study was consistent with biochemical data, suggesting an improvement in MSC(M)-treated group compared with PBS group. Transplantation of MSC(M) increased production of IL-4 and IFN-γ compared to PBS group.

Conclusion

Our results demonstrated that MSC(M) transplantation could facilitate recovery from chemically induced liver damage. Therefore, MSC(M) transplantation may represent a new concept for the treatment of liver diseases.

Keywords

Mice, liver damage, CCl4, bone marrow stromal cells, mesenchymal, cell transplantation.