ISSN 1866-8836
Клеточная терапия и трансплантация

Cellular Therapy and Transplantation is an international, peer-reviewed, Open Access journal which publishes articles in the fields of cellular and gene therapy and tissue transplantation. We welcome original research articles, review articles, short communications and clinical case reports. The major criteria for acceptance and publication of a manuscript are originality, high quality, scientific rigor, and interest to a wide audience of readers. The journal is issued in printed and digital version (www.cttjournal.com)

Editorial work, processing and publication of the articles are free of charge for the authors.

Papers accepted for publication in CTT are made available on this site at no charge to readers (Open Access). The articles published in CTT are provided under the following license: Creative Commons Attribution 3.0 Unported, http://creativecommons.org/licenses/by/3.0/ Authors retain the copyright of their contributions.
Volume 7, Number 4
12/27/2018
Volume 7, Number 4
Editor-in-Chief
Afanasyev B. V. (St. Petersburg, Russia)
Co-Editors-in-Chief
Wagemaker G. (Rotterdam, Netherlands)
Zander A. R. (Hamburg, Germany)
Deputy Editor
Fehse B. (Hamburg, Germany)
Managing Editor
Chukhlovin A. B. (St. Petersburg, Russia)
Editorial Board
Aleynikova O. V. (Minsk, Belarus)
Borset M. (Trondheim, Norway)
Chechetkin A. V. (St. Petersburg, Russia)
Fibbe W. (Leiden, Netherlands)
Galibin O. V. (St. Petersburg, Russia)
Hölzer D. (Frankfurt a.M., Germany)
Klimko N. N. (St. Petersburg, Russia)
Kolb H.-J. (München, Germany)
Kröger N. (Hamburg, Germany)
Kulagin A. D. (St. Petersburg, Russia)
Lange C. (Hamburg, Germany)
Mamaev N. N. (St. Petersburg, Russia)
Mikhailova N. B. (St. Petersburg, Russia)
Moiseev I. S. (St. Petersburg, Russia)
Nagler A. (Tel-Aviv, Israel)
Nemkov A. S. (St. Petersburg, Russia)
Paramonov I. V. (Kirov, Russia)
Roumiantsev A. G. (Moscow, Russia)
Savchenko V. G. (Moscow, Russia)
Smirnov A. V. (St. Petersburg, Russia)
Uss A. L. (Minsk, Belarus)
Zubarovskaya L. S. (St. Petersburg, Russia)
In this Issue
The ongoing issue of CTT opens with a review by Nouran Sabbagh and Axel R. Zander which deals with feasibility and relative efficiency of diff erent hematopoietic transplantation options and probable applications of CAR-T cells in treatment of multiple myeloma patients. In particular, various combinations of tandem hematopoietic transplants and donor lymphocyte infusions are discussed. A review article by Dr. Goloshchapov et al. concerns multiple factors infl uencing human gut microbiome in hematopoietic stem cell transplantation (HSCT), and prospects for fecal microbiota transplantation which now becomes a highly discutable topic. Special attention is drawn to eff ects of intensive therapy upon the intestinal microbiota parameters which may be of suffi cient prognostic value. An original clinical report by Dr. Ivan Moiseev et al. deals with risks of graft -versus-host disease factors in HSCT which arise with different modes of posttransplant immunosuppression. In case of cyclophosphamide-based treatment, cytomegalovirus seropositivity proved to be the major risk factor of GVHD, unlike several other risk factors found with conventional GVHD prophylaxis. A clinical report presented by Dr. Maxim Kucher et al. concerns the intensively studied effect of ABO and Rh- mismatch for the HSCT patients’ survival and subsequent complications. Th e study confi rmed common results on major ABO-incompatibility in the donor-recipient pairs and showed the Rh status (especially, ddccee constellation) to be a negative factor for the 1-year survival aft er HSCT. The available genetic markers of minimal residual disease, e.g., based on the Ig gene rearrangements, proved to be rather sensitive in relapse prediction and risk grouping among the children who underwent HSCT for acute lymphoblastic leukemia, as shown by Viktoria Lavrinenko et al. from the Minsk Center of Pediatric Oncology. Th is is a good example of clinical and laboratory consensus with valuable clinical outcome. An interesting clinical case was described by Dr. Omar Hamdy et al., who dealt with a kidney recipient who developed multiple cancers within several years of convential immunosuppressive therapy. In future, the DNA samples from such cases should be accumulated, requiring deep genetic studies searching for appropriate tumor risk genes. Experimental studies in this issue are presented by a timely report by Prof. Sergej Ulitovskiy et al. in rabbit model of bone injury treated with porous chitosan-based fi lling material which seems to be a biocompatible, bioinert, and bioresorbable matrix for local bone regeneration and fi brous replacement within 3-6 months post-injury. A small, but useful laboratory study by Dr. Oleg Glotov et al. aimed to compare the results of high-resolution HLA testing obtained by conventional Sanger method, and modern NGS-based assay. Th e data showed essential concordance, and a trend for lowering the NGS costs with increasing introduction of this approach in routine practice of Russian HLA-typing laboratories. Th e short report by Tatyana Parkhomenko et al. deals with a rarely used approach to in vitro testing of growth factors, i.e., mitochondria-mediated mechanisms of erythropoietin action upon lymhoid cells. At the current level of research, this technique may be used for fl ow cytometric screening of novel drugs and substances used in oncohematology.

Editorial article

The CTT story (2008-2018): 10 years of hopes and activities
Professor Boris V. Afanasyev, Editor-in-Chief, Cellular Therapy and Transplantation Journal

Review articles

Clinical studies

Different risk factors of acute and chronic graft-versus-host disease with conventional prophylaxis and posttransplantation cyclophosphamide in matched related and unrelated donor transplantations
Ivan S. Moiseev, Elena I. Darskaya, Tatyana A. Bykova, Elena V. Morozova, Alexander L. Alyanskiy, Elena V. Babenko, Sergey N. Bondarenko, Inna V. Markova, Boris V. Afanasyev
Impact of ABO- and Rh- incompatibility in allogeneic hematopoietic stem cell transplantation
Maxim A. Kucher 1, Dmitrii E. Pevtcov 1, Polina S. Kuga 1, Boris I. Smirnov 1,2, Alexander L. Alyanskiy 1, Natalia E. Ivanova 1, Maria A. Estrina 1, Elena V. Babenko 1, Burkhonidin B. Bakhovadinov 1, Ludmila S. Zubarovskaya 1, Boris V. Afanasyev 1
Minimal residual disease monitoring by RQPCR of Ig/TCR rearrangements: an effective method to predict relapse in children with acute lymphoblastic leukemia after allogeneic hematopoietic stem cell transplantation
Victoria A. Lavrinenko 1, Alexandr N. Meleshko 1, Dmitry V. Lutskovich 1, Yulia E. Mareiko 1, Dmitriy V. Prudnikov 1, Mikhail V. Belevtsev 1, Olga V. Aleynikova 1, Ildar M. Barkhatov 2, Boris V. Afanasyev 2

Clinical case

Synchronous skin squamous cell carcinoma and papillary thyroid carcinoma after renal transplantation: a case report
Omar Hamdy 1, Sara Raafat 2, Amr Abouzid 1, Mahmoud M. Saleh 1, Abdelhady M. Shebl 2

Experimental studies

Comparative analysis of NGS and Sanger sequencing methods for HLA typing at a Russian university clinic
Oleg S. Glotov 1,2, Olga V. Romanova 1,2, Yuri A. Eismont 1, Andrey M. Sarana 1,2, Sergey G. Scherbak 1,2, Elena V. Kuzmich 3, Alexander L. Alyanskiy 3, Natalya E. Ivanova 3, Vera V. Teplyashina 3, Yury A. Serov 3, Ludmila S. Zubarovskaya 3, Boris V. Afanasyev 3
Surgical treatment of inflammatory periodontal diseases using chitosan matrices
Sergey B. Ulitovskiy 1*, Anna V. Antipova 1*, Alexander D. Vilesov 1**, 2, GalinaYu. Yukina 1***, Dmitry N. Suslov 1****, 3, Pavel V. Popryadukhin 1****, 2, Oleg V. Galibin 1**
1* Department of Preventive Stomatology
1** Biotechnology Department, R.Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation
1*** Laboratory of Pathomorphology, Th e University Research Center
1**** Laboratory of Invasive Technologies, Th e University Research Center